One of the critical challenges of human neuron cultures is to keep them alive and
healthy for as long as they need to transition from neural progenitor cells to mature
neurons and neural networks. In brain organoids, human neurons grow isotropically
in three directions, creating a more natural and supporting environment for development,
which is reflected by the extended survival rate, the higher degree of structural
complexity, the improved cellular, and molecular composition, and the maturing electrophysiological
complexity. Several studies have shown that brain organoids can recapitulate critical
aspects of the developing brain [
[1]
]. Taking advantage of recent advances in genetic technologies and transcriptomics,
previous works provided a detailed characterization of cell types and developmental
states within organoids. As the field has started witnessing exponential growth, functional
analysis by means of electrophysiology studies has also become more frequently employed
to identify the biophysical phenotype of the cell population [
[2]
]. However, the functional characterization of 3D in vitro cultures remains exceptionally challenging.- Fair S.R.
- Julian D.
- Hartlaub A.M.
- Pusuluri S.T.
- Malik G.
- Summerfied T.L.
- Zhao G.
- Hester A.B.
- Ackerman W.E.
- Hollingsworth E.W.
- Ali M.
- McElroy C.A.
- Buhimschi I.A.
Electrophysiological Maturation of Cerebral Organoids Correlates with Dynamic Morphological
and Cellular Development.
Stem Cell Reports. 2020; 15: 855-868
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Bibliography
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- Electrophysiological Maturation of Cerebral Organoids Correlates with Dynamic Morphological and Cellular Development.Stem Cell Reports. 2020; 15: 855-868
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Article Info
Publication History
Accepted:
July 17,
2022
Received in revised form:
July 5,
2022
Received:
May 10,
2022
Publication stage
In Press Journal Pre-ProofIdentification
Copyright
© 2022 Published by Elsevier Inc on behalf of Society of Biological Psychiatry.
