In 2011, Moreau et al. (
1
) reported in Biological Psychiatry findings concerning patterns of microRNA (miRNA) expression in postmortem samples
from prefrontal cortex (Brodmann area 9) from the Stanley Medical Research Institute
(SMRI) (Chevy Chase, Maryland). They used advanced statistical techniques and miRNA
assays by real-time quantitative polymerase chain reaction (
2
) (TaqMan probes; Applied Biosystems, Foster City, California) for the canonical sequences
in early versions of miRBase (http://www.mirbase.org/) (
3
). All demographics, sample processing, and data analysis steps were carefully described
in their article. The main conclusion was that certain miRNAs were distinguished in
comparisons of samples from unaffected control subjects versus samples from subjects
with schizophrenia and versus samples from subjects with bipolar disorder. They identified
24 miRNAs in particular with distinctive fold changes [Figure 1 in Moreau et al. (
1
)].To read this article in full you will need to make a payment
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References
- Altered microRNA expression profiles in postmortem brain samples from individuals with schizophrenia and bipolar disorder.Biol Psychiatry. 2011; 69: 188-193
- Real-time quantification of microRNAs by stem-loop RT-PCR.Nucleic Acids Res. 2005; 33: e179
- miRBase: Annotating high confidence microRNAs using deep sequencing data.Nucleic Acids Res. 2014; 42: D68-D73
- MicroRNA-132 dysregulation in schizophrenia has implications for both neurodevelopment and adult brain function.Proc Natl Acad Sci U S A. 2012; 109: 3125-3130
- MicroRNA expression profiling in the prefrontal cortex of individuals affected with schizophrenia and bipolar disorders.Schizophr Res. 2010; 124: 183-191
- Policy: NIH plans to enhance reproducibility.Nature. 2014; 505: 612-613
Article info
Publication history
Published online: November 28, 2015
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© 2016 Society of Biological Psychiatry.
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Access this article on ScienceDirectLinked Article
- Altered MicroRNA Expression Profiles in Postmortem Brain Samples from Individuals with Schizophrenia and Bipolar DisorderBiological PsychiatryVol. 69Issue 2
- Reply to: Reproducibility and Visual Inspection of DataBiological PsychiatryVol. 80Issue 5
- PreviewThe correspondence from Jeffries and Perkins (1) raises an important potential limitation of data repositories. Our original publication (2) used tissue from the Stanley Medical Research Institute (SMRI) Array Collection (Chevy Chase, Maryland). According to the terms of use of this tissue, samples were provided for analysis blind to diagnosis and other clinical and laboratory variables. Raw data were returned to the SMRI before the code linking samples to the descriptive data was provided. The data analyzed in the correspondence from Jeffries and Perkins appear to be these raw data, presumably obtained directly from the SMRI database (individual subject data were not included as supplemental data in our 2011 publication, and we have not provided it to anyone other than the SMRI).
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