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Reproducibility and Visual Inspection of Data

Published:November 28, 2015DOI:https://doi.org/10.1016/j.biopsych.2015.07.029
      In 2011, Moreau et al. (
      • Moreau M.P.
      • Bruse S.E.
      • David-Rus R.
      • Buyske S.
      • Brzustowicz L.M.
      Altered microRNA expression profiles in postmortem brain samples from individuals with schizophrenia and bipolar disorder.
      ) reported in Biological Psychiatry findings concerning patterns of microRNA (miRNA) expression in postmortem samples from prefrontal cortex (Brodmann area 9) from the Stanley Medical Research Institute (SMRI) (Chevy Chase, Maryland). They used advanced statistical techniques and miRNA assays by real-time quantitative polymerase chain reaction (
      • Chen C.
      • Ridzon D.A.
      • Broomer A.J.
      • Zhou Z.
      • Lee D.H.
      • Nguyen J.T.
      • et al.
      Real-time quantification of microRNAs by stem-loop RT-PCR.
      ) (TaqMan probes; Applied Biosystems, Foster City, California) for the canonical sequences in early versions of miRBase (http://www.mirbase.org/) (
      • Kozomara A.
      • Griffiths-Jones S.
      miRBase: Annotating high confidence microRNAs using deep sequencing data.
      ). All demographics, sample processing, and data analysis steps were carefully described in their article. The main conclusion was that certain miRNAs were distinguished in comparisons of samples from unaffected control subjects versus samples from subjects with schizophrenia and versus samples from subjects with bipolar disorder. They identified 24 miRNAs in particular with distinctive fold changes [Figure 1 in Moreau et al. (
      • Moreau M.P.
      • Bruse S.E.
      • David-Rus R.
      • Buyske S.
      • Brzustowicz L.M.
      Altered microRNA expression profiles in postmortem brain samples from individuals with schizophrenia and bipolar disorder.
      )].
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      References

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        • Bruse S.E.
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        Altered microRNA expression profiles in postmortem brain samples from individuals with schizophrenia and bipolar disorder.
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      Linked Article

      • Altered MicroRNA Expression Profiles in Postmortem Brain Samples from Individuals with Schizophrenia and Bipolar Disorder
        Biological PsychiatryVol. 69Issue 2
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          MicroRNAs (miRNAs) are potent regulators of gene expression with proposed roles in brain development and function. We hypothesized that miRNA expression profiles are altered in individuals with severe psychiatric disorders.
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      • Reply to: Reproducibility and Visual Inspection of Data
        Biological PsychiatryVol. 80Issue 5
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          The correspondence from Jeffries and Perkins (1) raises an important potential limitation of data repositories. Our original publication (2) used tissue from the Stanley Medical Research Institute (SMRI) Array Collection (Chevy Chase, Maryland). According to the terms of use of this tissue, samples were provided for analysis blind to diagnosis and other clinical and laboratory variables. Raw data were returned to the SMRI before the code linking samples to the descriptive data was provided. The data analyzed in the correspondence from Jeffries and Perkins appear to be these raw data, presumably obtained directly from the SMRI database (individual subject data were not included as supplemental data in our 2011 publication, and we have not provided it to anyone other than the SMRI).
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