ABSTRACT
Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS)
(MALDI-IMS) provides a technical means for simultaneous analysis of precise anatomic
localization and regulation of peptides. We explored the technical capability of matrix-assisted
laser desorption ionization mass spectrometry for characterization of peptidomic regulation
by an addictive substance along two distinct projection systems in the mouse striatum.
The spatial expression patterns of substance P and proenkephalin, marker neuropeptides
of two distinct striatal projection neurons, were negatively correlated at baseline.
We detected 768 mass/charge (m/z) peaks whose expression levels were mostly negatively
and positively correlated with expression levels of substance P and proenkephalin
A (amino acids 218–228), respectively, within the dorsal striatum. After nicotine
administration, there was a positive shift in correlation of mass/charge peak expression
levels with substance P and proenkephalin A (218–228). Our exploratory analyses demonstrate
the technical capacity of MALDI-IMS for comprehensive identification of peptidomic
regulation patterns along histochemically distinguishable striatal projection pathways.
Keywords
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Article info
Publication history
Published online: September 25, 2015
Accepted:
September 18,
2015
Received:
July 15,
2015
Identification
Copyright
© 2016 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.
