Background
Major depressive disorder (MDD) is inconsistently associated with elevations in proinflammatory
cytokines and neuropeptides. We used a skin sweat patch, recently validated in healthy
control subjects, and recycling immunoaffinity chromatography to measure neuroimmune
biomarkers in patients with MDD mostly in remission.
Methods
We collected blood at 8:00 am and applied skin sweat patches for 24 hours in 21- to 45-year-old premenopausal women
(n = 19) with MDD (17/19 in remission) and age-matched healthy controls (n = 17) participating in the POWER (Premenopausal, Osteopenia/Osteoporosis, Women,
Alendronate, Depression) Study.
Results
Proinflammatory cytokines, neuropeptide Y, substance P, and calcitonin-gene-related
peptide were significantly higher and vasoactive intestinal peptide, a marker of parasympathetic
activity, was significantly lower in patients compared to controls, and depressive
symptomatology strongly correlated with biomarker levels. All analytes were strongly
correlated in the skin sweat patch and plasma in patients (r = .73 to .99; p < .0004).
Conclusions
The skin sweat patch allows detection of disrupted patterns of proinflammatory cytokines
and neuropeptides in women with MDD in clinical remission, which could predispose
to medical consequences such as cardiovascular disease, osteoporosis, and diabetes.
This method permits measurement of cytokines in ambulatory settings where blood collection
is not feasible.
Key Words
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Article info
Publication history
Published online: July 28, 2008
Accepted:
May 30,
2008
Received in revised form:
May 19,
2008
Received:
October 19,
2007
Identification
Copyright
Published by Elsevier Inc.
