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Research Article| Volume 34, ISSUE 12, P824-838, December 15, 1993

Olfactory neuroblasts from Alzheimer donors: Studies on APP processing and cell regulation

DOI:https://doi.org/10.1016/0006-3223(93)90051-E
Olfactory neuroblasts from Alzheimer donors: Studies on APP processing and cell regulation
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      Abstract

      Cell lines of continuously dividing human olfactory neuroblasts can be propagated using olfactory epithelium obtained from human donors at biopsy or autopsy. The expression of neuronal proteins in these cells, such as neurofilament protein and tau proetin, can be increased using a combination of factors including nerve growth factor, fibroblast growth factor, interleukin 1 and interleukin 6. These cells also express aspects of human disease. Olfactory neuroblasts generated from donors with the common, sporadic forms of Alzheimer's disease, show elevated levels of the direct precursor to β-amyloid, the amyloid precursor protein C-terminal derivative (CTD). When treated with the lysosomal inhibitor chloroquine, immunoblots of Alzheimer olfactory neuroblasts show seven-fold higher levels of CTDs than immunoblots from age-matched control neuroblasts. The disease related increases in CTDs can be reversed by treatment with agents that increase intracellular cyclic adenosine monophosphate (cAMP), such as dibutyril-cyclic-AMP, theophylline, and isoproteronol.

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      Article info

      Publication history

      Received in revised form: October 14, 1993
      Received: June 2, 1993

      Footnotes

      ☆This research was supported in part through a cooperative research and development agreement with Molecular Geriatrics Corp. (Lake Bluff, IL).

      Identification

      DOI: https://doi.org/10.1016/0006-3223(93)90051-E

      Copyright

      © 1993 Published by Elsevier Inc.

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