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Research Article| Volume 30, ISSUE 3, P269-282, August 01, 1991

Alterations of local cerebral blood flow and glucose uptake by electroconvulsive shock in rats

  • Amiram I. Barkai
    Correspondence
    Address reprint requests to Dr. Amiram I. Barkai, Box 54, New York State Psychiatric Institute, 722 West 168th Street, New York, N.Y. 10032.
    Affiliations
    From the New York State Psychiatric Institute, College of Physicians and Surgeons, Columbia University, New York, NY, USA

    Department of Psychiatry, College of Physicians and Surgeons, Columbia University, New York, NY, USA
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  • Isak Prohovnik
    Affiliations
    From the New York State Psychiatric Institute, College of Physicians and Surgeons, Columbia University, New York, NY, USA

    Department of Psychiatry, College of Physicians and Surgeons, Columbia University, New York, NY, USA

    Departments of Neurology and Radiology, College of Physicians and Surgeons, Columbia University, New York, NY, USA
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  • William L. Young
    Affiliations
    Department of Anesthesiology, College of Physicians and Surgeons, Columbia University, New York, NY, USA
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  • Margaret Durkin
    Affiliations
    From the New York State Psychiatric Institute, College of Physicians and Surgeons, Columbia University, New York, NY, USA
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  • Henry D. Nelson
    Affiliations
    From the New York State Psychiatric Institute, College of Physicians and Surgeons, Columbia University, New York, NY, USA
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Open AccessDOI:https://doi.org/10.1016/0006-3223(91)90111-X
Alterations of local cerebral blood flow and glucose uptake by electroconvulsive shock in rats
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      Abstract

      The effects of single and repeated electroconvulsive shock (ECS) treatments on regional cerebral blood flow (rCBF) and on rates of glucose flow from blood to local brain areas (rCGF), were investigated in pentobarbital-anesthetized rats, using quantitative autoradiographic techniques. Effects of single ECS on rCBF were assessed at two average time points of 15 and 55 sec after the application of the electric current, whereas the effects on rCGF were assessed at 70 and 110 sec. Effects of repeated ECS were assessed 24 hr after the last ECS in a series of eight daily treatments. Single ECS caused marked increases in rCGF in different brain structures, but no significant effects were observed after repeated ECS. Similarly, substantial increases in rCBF were seen during and immediately after the ECS-induced seizure, but not 24 hr after the last treatment of repeated ECS. Single ECS appeared to have differential effects on rCBF in hind-brain structures as compared to more anterior regions. A linear relationship between rCBF and rCGF values was established in control animals, indicating coupling of these two variables with a constant rCBF/rCGF ratio. ECS caused an apparent increase in the CBF/CGF ratio, which might be attributed to the different temporal resolution of the two methods used here to estimate rCGF and rCBF. Analysis of the increments of rCGF and rCBF extrapolated to the same point in time after a single ECS (10 sec), revealed that in many of the examined structures the CBF/CGF ratio was similar to that observed in control animals, indicating that the coupling of CBF and CGF is maintained during the seizure. But in some brain stem structures such as the dorsal raphe, inferior colliculi, superior olivary nucleus, and the vestibular nucleus there were large increases in CGF associated with a marked drop in the CBF/CGF ratio. This observation suggests that high metabolic demands can be met by increased local blood flow up to a given “ceiling” keeping the glucose clearance from blood to brain tissue constant. However, when the metabolic demands exceed this upper limit, the additional demands could be met by an increased clearance of glucose without a change in CBF.

      References

        • Ackermann R.F.
        • Engel J.
        • Baxter L.
        Positron emission tomography and autoradiographic studies of glucose utilization following electroconvulsive seizures in humans and rats.
        Ann NY Acad Sci. 1986; 462: 263-269
        • Baker N.
        • Huebotter R.J.
        Compartmental and semicompartmental approaches for measuring glucose carbon flux to fatty acids and other products in vivo.
        J Lipid Res. 1972; 13: 716-724
        • Balazs R.
        Carbohydrate metabolism.
        in: Handbook of Neurochemistry. Vol 3. Plenum Press, New York1969: 1-36
        • Barkai A.I.
        Interaction of drugs and electroshock treatment on cerebral monoaminergic systems.
        Ann NY Acad Sci. 1986; 462: 147-162
        • Barkai A.I.
        • Durkin M.
        • Nelson H.D.
        Localized alterations of dopamine receptor binding in rat brain by repeated electroconvulsive shock: An autoradiographic study.
        Brain Res. 1990; 529: 208-213
        • Barkai A.I.
        • Mahadik S.
        • Rapport M.M.
        Flow in vivo of glucose carbon to brain proteins in rats: Effect of starvation.
        J Neurochem. 1974; 22: 511-516
        • Biegon A.
        • Israeli M.
        Localization of the effects of ECS on beta receptor binding sites in the rat brain.
        Eur J Pharmacol. 1986; 123: 329-334
        • Biegon A.
        • Israeli M.
        Quantitative autoradiographic analysis of the effects of ECS on 5HT2 receptors in male and female rats.
        J Neurochem. 1987; 48: 1386-1391
        • Charney D.S.
        • Menkes D.B.
        • Phil M.
        • Heninger G.R.
        Receptor sensitivity and the mechanism of action of antidepressant treatment. Implication for the ethiology and therapy of depression.
        Arch Gen Psychiatry. 1981; 38: 1160-1180
        • Cremer J.E.
        • Ray D.E.
        • Sarna G.S.
        • Cunningham V.J.
        A study of the kinetic behavior of glucose based on simultaneous estimations of the influx and phosphorylation in brain regions of rats in different physiological states.
        Brain Res. 1981; 221: 331-342
        • Dunnett C.W.
        A multiple comparison procedure for comparing several treatments with a control.
        J Am Stat Assoc. 1955; 50: 1096-1121
        • Fink M.
        Efficacy and safety of induced seizures (EST) in man.
        Comp Psychiatry. 1978; 19: 1-18
        • Fox P.T.
        • Reichle M.E.
        Focal physiological uncoupling of cerebral blood flow and oxidative metabolism during somatosensopry stimulation in human subjects.
        in: Proc Natl Acad Sci (USA). 83. 1986: 1140-1144
        • Hawkins R.A.
        • Miller A.L.
        • Cremer J.E.
        • Veech R.L.
        Measurement of the rate of glucose utilization by rat brain in vivo.
        J Neurochem. 1974; 23: 917-923
        • Hawkins R.A.
        • Mans A.M.
        • Davis D.W.
        • Hibbard L.S.
        • Lu D.M.
        Glucose availability to individual cerebral structures is correlated to glucose metabolism.
        J Neurochem. 1983; 40: 1013-1018
        • Horton R.W.
        • Meldrum B.S.
        • Padley T.A.
        • McWilliam J.R.
        Regional cerebral blood flow in the rat during prolonged seizure activity.
        Brain Res. 1980; 192: 399-412
        • Ingvar M.
        • Siesjo B.K.
        Local blood flow and glucose metabolism in the rat brain during sustained bicuculline-induced seizures.
        Acta Neurol Scand. 1983; 68: 129-144
        • Lou H.C.
        • Edvinsson L.
        • MacKenzie E.T.
        The concept of coupling blood flow to brain function: Revision required?.
        Ann Neurol. 1987; 22: 289-297
        • Lu D.M.
        • Davis D.W.
        • Mans A.M.
        • Hawkins R.A.
        Regional cerebral glucose utilization measures with [14C]-glucose in brief experiments.
        Am J Physiol. 1983; 245: C428-C438
      20. Malitz S. Sackeim H.A. Electroconvulsive therapy: Clinical and basic research issues. Ann NY Acad Sci. 462. 1986: 424
        • Medical Research Council
        Clinical trial of the treatment of depressive illness.
        Br Med J. 1965; 1: 886-888
        • Meldrum B.S.
        • Nilsson B.
        Cerebral blood flow and metabolic rate early and late in prolonged epileptic seizures induced in rats by bicuculline.
        Brain. 1976; 99: 523-542
        • Nilsson B.
        • Rehncrona S.
        • Siesjo B.K.
        Coupling of cerebral metabolism and blood flow in epileptic seizures, hypoxia and hyperglycemia.
        in: CIBA Foundation Symp. Cerebral Vascular Smooth Muscle and Its Control. 56. Elsevier, New York1978: 199-214
        • Orzi F.
        • Passarelli F.
        • Diana G.
        • Fieschi C.
        Effects of single and repeated ECS on local cerebral glucose utilization in the conscious rat.
        Brain Res. 1987; 423: 144-148
        • Perumal A.S.
        • Barkai A.I.
        Beta-adrenergic receptor binding in different regions of rat brain after various intensities of electroshock: Relationship to postictal EEG.
        J Neurosci Res. 1982; 7: 289-296
        • Prohovnik I.
        • Sackeim H.A.
        • Decina P.
        • Malitz S.
        Acute reductions of regional cerebral blood flow following ECT.
        Ann NY Acad Sci. 1986; 462: 249-262
        • Pulsinelli W.A.
        • Levy D.E.
        • Duffy T.E.
        Regional cerebral blood flow and glucose metabolism following transient forebrain ischemia.
        Ann Neurology. 1982; 11: 499-509
        • Roy C.W.
        • Sherrington C.S.
        On the regulation of the blood supply of the brain.
        J Physiol (Lond). 1890; 11: 85-108
        • Sacks W.
        • Sacks S.
        • Badalamenti A.
        • Fleischer A.
        A proposed method for the determination of cerebral regional intermediary glucose metabolism in humans in vivo using specifically labelled [11C]-glucose and PETT. 1. An animal model with [14C]-glucose and rat brain autoradiography.
        J Neurosci Res. 1982; 7: 57-69
        • Sokoloff L.
        Relationships among local functional activity, energy metabolism and blood flow in the central nervous system.
        in: Fed Proc. 40. 1981: 2311-2316
        • Sokoloff L.
        • Reivich M.
        • Kennedy C.
        • et al.
        The [14C]-deoxyglucose method for the measurement of local cerebral glucose utilization: theory procedure and normal values in the conscious and anesthetized albino rat.
        J Neurochem. 1977; 28: 897-916
        • Sulser F.
        Serotonin-norepinephrine receptor interactions in the brain: Implications for the pharmacology and pathophysiology of affective disorders.
        J Clin Psychiatry. 1987; 48: 12-18
        • Young W.L.
        • Josovitz K.
        • Morales O.
        • Chien S.
        The effect of Nimodipine on postischemic cerebral glucose utilization and blood flow in the rat.
        Anesthesiology. 1987; 57: 54-59

      Article info

      Publication history

      Received in revised form: March 30, 1991
      Received: December 13, 1990

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      DOI: https://doi.org/10.1016/0006-3223(91)90111-X

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      © 1991 Published by Elsevier Inc.

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