This paper is only available as a PDF. To read, Please Download here.
A new assay technique for the determination of neurotransmitter binding in retinal fragments has been used to characterize and quantify beta-adrenergic receptors with the ligand [3H]CGP-12177. This assay allowed us to quantify beta-adrenergic receptors in the retina, pineal gland, and hypothalamus obtained from individual rats during a 10-hr period around the switch from light to dark under a 12-hr light/12-hr dark lighting cycle. A significant rhythm of beta-adrenergic binding was observed in the retina and pineal gland. These rhythms were abolished by chronic lithium treatment. In contrast to previous observations in whole brain preparations, lithium did not affect beta-adrenergic binding in brain tissue (hypothalamus) using this assay. Our data suggest that lithium may attenuate beta-adrenergic receptor down-regulation in pineal and retinal tissue. To the extent that this mechanism is important for the coding of information about light and dark in the environment, these observations might assist in our understanding of the clinical chronopharmacological properties reported for lithium.
To read this article in full you will need to make a payment
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Biological Psychiatry
Already a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
- Unidirectionality of lithium stabilization of adrenergic and cholinergic receptors.in: Emrich HM Aldenhoff JB Lux HD Basic Mechanisms of Action of Lithium. Excerpta Medica, Amsterdam1982: 146-153
- The daily light-dark cycle and rhythmic metabolism in the photoreceptor-pigment epithelial complex.Prog Ret Res. 1982; 1: 81-124
- Regulation and possible role of serotonin N-acetyl-transferase in the retina.in: Fed Proc. 43. 1984: 2704-2708
- N-acetyltransferase activity responds to environmental lighting in the eyes as well as in the pineal.Nature. 1979; 281: 479-481
- Lithium does not prevent ECS induced decreases in beta-adrenergic receptors.Psychopharmacology. 1982; 78: 190-191
- Studies on melatonin and other pineal factors.in: Clinical Neuroendocrinology. vol 2. Academic Press, Orlando, FL1982: 205-264
- Melatonin in the retina and the Harderian gland.Exp Eye Res. 1978; 27: 323-333
- Hamster and rat pineal gland beta-adrenoreceptor characterization with iodocyanopindolol and the effect of decreased catecholamine synthesis on the receptor.J Pineal Res. 1985; 2: 51-66
- Light-dependent regulation of dopamine receptors in mammalian retina.Brain Res. 1985; 335: 321-325
- Pineal melatonin levels and beta-receptor density: Changes associated with puberty in the male rat.Adv Biosci. 1985; 53: 139-144
- The effect of chronic lithium treatment on rat pineal N-acetyltransferase rhythms.J Pharmacol Exp Ther. 1981; 219: 121-124
- Melatonin synthesis by the retina.Science. 1979; 204: 183-184
- 24-Hour rhythm of hypothalamic melatonin immunoflourescence correlates with serum and retinal melatonin rhythms.Neuroendocrinology. 1982; 34: 363-368
- Catecholamine systems of retina: A model for studying synaptic mechanisms.Life Sci. 1984; 35: 1135-1147
- Chronic lithium prevents reserpine-induced supersensitivity of adenylate cyclase.J Pharm Pharmacol. 1980; 32: 510-511
- Examples of homospecific and heterospecific receptor regulation.TIPS. 1985; 6: 242-245
- Catecholamines and indoleamines in retina.in: Fed Proc. 43. 1984: 2697-2698
- Alpha2-adrenergic receptors influence tyrosine hydroxylase activity in retinal dopamine neurons.Life Sci. 1983; 33: 2455-2463
- Receptor binding in intact rat retina: Characterization of beta-adrenergic ([3H]CGP-12177) and opiate ([3H]naloxone) binding sites in vitro.J Physiol. 1986; 376: 22p
- The effect of antidepressants on circadian rhythms in brain neurotransmitter receptors.Acta Pharmacol Toxicol. 1985; 56: 162-169
- Effect of lithium on circadian neurotransmitter receptor rhythms.Neuropsychobiology. 1982; 8: 41-50
- Modulation of receptor sensitivity in the pineal: Roles of cyclic nucleotides.in: Soc Neurosci Symp. II. 1977: 376-398
- Postsynaptic beta-adrenergic receptors potentiate the beta-adrenergic stimulation of pineal serotonin N-acetyltransferase.in: Proc Natl Acad Sci USA. 80. 1983: 599-603
- Retinal pigment epithelium in culture demonstrates a distinct beta-adrenergic receptor.Exp Eye Res. 1984; 38: 7-13
- Hypothalamic alpha2- and beta-adrenoceptor rhythms are correlated with circadian feeding: Evidence from chronic methamphetamine treatment and withdrawal.Brain Res. 1984; 321: 83-90
- Central neural mechanisms in diurnal rhythm regulation and neuroendocrine response to light.Psychoneuroendocrinology. 1976; 12: 265-279
- Melatonin in the human retina.Exp Eye Res. 1984; 38: 213-215
- Determination of N-acetyl-serotonin and melatonin activities in the pineal gland, retina, Harderian gland, brain and serum of rats and chickens.Neuroendocrinology. 1977; 23: 1-13
- Usdin E Catecholamines: Basic and Clinical Frontiers. Pergamon Press, New York1979: 583
- Rhythms in immunoreactive melatonin in the retina and harderian gland of rats: Persistence after pinealectomy.Life Sci. 1983; 32: 1229-1236
- Circadian cycles in binding of [3H]-alprenolol to beta-adrenergic receptor sites in rat pineal.Nature. 1975; 258: 435-436
- The effect of imipramine and lithium on alpha- and beta-receptor binding in rat brain.Brain Res. 1979; 160: 186-191
- Melatonin, the retinal-hypothalamic-pineal axis and circadian rhythm regulation.in: Johnson FN Lithium Therapy Monographs, vol 2, Lithium and the Endocrine System. Karger, Basel1987 (in press).
- Twenty-four hour resting prolactin levels in male rats: The effect of septal lesions and order of sacrifice.Endocrinology. 1976; 98: 1516-1522
- Lithium and melatonin rhythms: Implications for depression.Adv Biosci. 1985; 53: 333-338
- Lithium and melatonin in pigmented eye rats: Effects of dose and time of day.Prog Neuropsychopharmacol Biol Psychiatry. 1984; 8: 732-736
- Lithium and circadian patterns of melatonin in the retina, hypothalamus, pineal and serum.Prog Neuropsychopharmacol Biol Psychiatry. 1986; (in press).
- Lithium effects on adrenergic receptor supersensitivity in rat brain.Eur J Pharmacol. 1979; 58: 85-86
- Lithium in pigmented eye rats: Effects of dose and time of day on drinking, body weight, retinal and blood distribution.Prog Neuropsychopharmacol Biol Psychiatry. 1984; 8: 765-768
- Beta-adrenergic [3H]CGP-12177) binding to brain slices and single intact pineal glands.Neurochem Res. 1985; 10: 829-839
- Tissue slice in radioligand binding assays: Studies in brain, pineal and muscle.Life Sci. 1986; 39: 2037-2048
- Antidepressant drugs: Effects on the circadian system.in: Wehr TA Goodwin FK Circadian Rhythms in Psychiatry. Boxwood Press, Pacific Grove, CA1983: 235-264
- Effect of chronic lithium treatment on rat pineal rhythms: N-acetyltransferase, N-acetylserotonin and melatonin.J Pharmacol Exp Ther. 1983; 226: 733-737
- Lithium does not prevent agonist-induced subsensitivity of human adenylate-cyclase.Biol Psychiatry. 1982; 17: 343-350
Received in revised form: October 13, 1986
Received: April 28, 1986We are indebted to Professor E.V. YoungLai, who provided laboratory support for part of this work.
© 1987 Published by Elsevier Inc.