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Research Article| Volume 22, ISSUE 10, P1191-1200, October 1987

Lithium and rhythms of beta-adrenergic ([3H]CGP-12177) binding in intact rat retina, pineal gland, and hypothalamus

  • Michael Wilkinson
    Footnotes
    Affiliations
    From the Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova ScotiaCanada

    From the Department of Obstetrics and Gynecology, Dalhousie University, Halifax, Nova ScotiaCanada
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  • Mahendra Joshi
    Affiliations
    From the Department of Neurosciences, McMaster University, Hamilton, OntarioCanada
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  • Eva S. Werstiuk
    Footnotes
    Affiliations
    From the Department of Neurosciences, McMaster University, Hamilton, OntarioCanada
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  • Jo Seggie
    Correspondence
    Address reprint requests to Dr. Jo Seggie, Department of Neurosciences, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada
    Footnotes
    Affiliations
    From the Department of Psychiatry, McMaster University, Hamilton, Ontario, Canada

    From the Department of Psychiatry, University of Western Ontario, London, Ontario, Canada

    From the Department of Neurosciences, McMaster University, Hamilton, OntarioCanada

    From the Department of Psychiatry, University of Western Ontario, London, Ontario, Canada

    From the Department of Psychiatry, McMaster University, Hamilton, Ontario, Canada
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  • Author Footnotes
    1 Supported in part by the Canadian MRC are recipients of OMHF career awards.
    2 Supported in part by the Ontario Mental Health Foundation are recipients of OMHF career awards.
DOI:https://doi.org/10.1016/0006-3223(87)90027-8
Lithium and rhythms of beta-adrenergic ([3H]CGP-12177) binding in intact rat retina, pineal gland, and hypothalamus
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      Abstract

      A new assay technique for the determination of neurotransmitter binding in retinal fragments has been used to characterize and quantify beta-adrenergic receptors with the ligand [3H]CGP-12177. This assay allowed us to quantify beta-adrenergic receptors in the retina, pineal gland, and hypothalamus obtained from individual rats during a 10-hr period around the switch from light to dark under a 12-hr light/12-hr dark lighting cycle. A significant rhythm of beta-adrenergic binding was observed in the retina and pineal gland. These rhythms were abolished by chronic lithium treatment. In contrast to previous observations in whole brain preparations, lithium did not affect beta-adrenergic binding in brain tissue (hypothalamus) using this assay. Our data suggest that lithium may attenuate beta-adrenergic receptor down-regulation in pineal and retinal tissue. To the extent that this mechanism is important for the coding of information about light and dark in the environment, these observations might assist in our understanding of the clinical chronopharmacological properties reported for lithium.
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      Article info

      Publication history

      Received in revised form: October 13, 1986
      Received: April 28, 1986
      We are indebted to Professor E.V. YoungLai, who provided laboratory support for part of this work.

      Identification

      DOI: https://doi.org/10.1016/0006-3223(87)90027-8

      Copyright

      © 1987 Published by Elsevier Inc.

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