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Abstract
A new assay technique for the determination of neurotransmitter binding in retinal
fragments has been used to characterize and quantify beta-adrenergic receptors with
the ligand [3H]CGP-12177. This assay allowed us to quantify beta-adrenergic receptors in the retina,
pineal gland, and hypothalamus obtained from individual rats during a 10-hr period
around the switch from light to dark under a 12-hr light/12-hr dark lighting cycle.
A significant rhythm of beta-adrenergic binding was observed in the retina and pineal
gland. These rhythms were abolished by chronic lithium treatment. In contrast to previous
observations in whole brain preparations, lithium did not affect beta-adrenergic binding
in brain tissue (hypothalamus) using this assay. Our data suggest that lithium may
attenuate beta-adrenergic receptor down-regulation in pineal and retinal tissue. To
the extent that this mechanism is important for the coding of information about light
and dark in the environment, these observations might assist in our understanding
of the clinical chronopharmacological properties reported for lithium.
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Article info
Publication history
Received in revised form:
October 13,
1986
Received:
April 28,
1986
We are indebted to Professor E.V. YoungLai, who provided laboratory support for part of this work.Identification
Copyright
© 1987 Published by Elsevier Inc.
