Research Article| Volume 22, ISSUE 8, P987-994, August 1987

Temperature dependence of the molecular dynamics of platelet membranes in alzheimer's disease

  • George S. Zubenko
    Address reprint requests to Dr. George S. Zubenko, Western Psychiatric Institute and Clinic, E-1231, 3811 O'Hara Street, Pittsburgh, PA 15213.
    Western Psychiatric Institute and Clinic and the Department of Psychiatry, University of Pittsburgh School of Medicine, PittsburghUSA

    Department of Biological Sciences, Mellon Institute, Carnegie-Mellon University, PittsburghUSA
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  • Ivana Malinakova
    Western Psychiatric Institute and Clinic and the Department of Psychiatry, University of Pittsburgh School of Medicine, PittsburghUSA
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  • Bruce M. Cohen
    Mailman Research Center, McLean Hospital, Belmont, MAUSA
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      The biophysical properties of platelet membranes from five patients with Alzheimer-type dementia and five neurologically healthy controls, matched for age and sex, were examined by fluorescence spectroscopy employing the fluorescent probes DPH and TMADPH. The anisotropy of DPH has previously been observed to be reduced in platelet membranes of patients with Alzheimer-type dementia. However, Alzheimer's disease was not associated with a significant change in the flow activation energy at either deep or superficial regions of platelet membranes. In addition, the temperature-dependent phase transition observed with TMADPH occurred at the same mean temperature in platelet membranes from the demented and control groups. Supported by Alzheimer Disease Research Center Grant AG05133 (G.S.Z): Mental Health Clinical Research Center Grant MH30915 (G.S.Z.); project grant MH38313 (B.M.C.); and the program grants AG03705 (G.S.Z.), MH36224 (B.M.C.), and MH31154 (B.M.C.). G.S.Z. was the recipient of ADAMHA Research Scientist Development Award MH00540.
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